PhiC31 transformation services
(Please read below, service terms included)
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Service Type
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Price (CNY)
|
Description
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1550
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PhiC31 Basic - injection only (ship larvae only) | |
|
1880
|
PhiC31 Standard - Plan G plus generation of w+ or y+ transformant (ship transformant) | |
|
2100
|
PhiC31 Premium - Plan H plus balancing (ship balanced lines) | |
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2210
|
Plan I plus an early/partial shipment of Plan H, i.e. ship Plan H first (this shipment may not contain all lines), then regular Plan I shipment when finish |
Plan G - PhiC31 Basic Service (CNY1550): Similar to the described Plan A. This is an injection-only service and the cost is based on injecting 200 attP embryos. Usually the G0 embryos will be collected from the crosses between your selected attP strain and the PhiC31-source strain. For those strains already contain the PhiC31 source, the G0 embryos will be collected directly. The injected G0 larvae will be shipped to you and you are responsible for identifying your own transformants and remove the PhiC31 source (by phenotype). You will pay full amount regardless of the number of transformants found in your lab.
There will be no free second injection in many situations including shipping mistake by the courier.
Plan H - PhiC31 Standard Service (CNY1880): Similar to the described Plan B and the procedures for Plan G. This service includes injection, generation and identification of transformants (w+ or y+ included as priced; other markers refer to the "others and promotions" price table). We will inject your attB DNA sample into 200 embryos, back-cross SELECTED survival G0 adults to yw, expand G1 transformants by crossing to yw again, and ship only the G2 transformants to you.
When using this service, we will try to get at least 1 independent transformant for a reasonably sized construct (smaller than 13 kb) due to the fact that in many cases you only need one line to perform your experiment. We will send you maximum 5 lines if there are more found in the screening process.
We will take the same measure and charge in the same way for the special situations (i.e. toxic or failure transformation) as described in Plan B for construct smaller than 13 kb. For constructs larger than 13 kb, we will charge you full amount regardless of the transformation result.
Plan I - PhiC31 Premium Service (CNY2100): Similar to the described Plan C. This service includes injection, generation, identification of transformants and balancing. We will balance the transformants in addition to the Plan H service.
We send you maximum 5 balanced transformants.
We will take the same measure and charge in the same way for the special situations (i.e. toxic or failure transformation) as described in Plan H.
Plan H+I - Service (CNY2210): Plan I plus an early/partial shipment of Plan H. i.e. ship Plan H first (this shipment may not contain all lines), then regular Plan I shipment when finish.
